Difference between revisions of "Part:BBa K3600002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | . | + | The sequence of 500bp preceding the ORF of YCF1 was retrieved from the yeast genome database. No modifications were applied. |
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===Source=== | ===Source=== | ||
− | + | Promoter of the vacuolar glutathione S-conjugate transporter (YDR135C) from the genome of S. Cerevisiae. | |
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===References=== | ===References=== |
Latest revision as of 18:45, 14 June 2020
YCF1 promoter part plasmid
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 935
Illegal EcoRI site found at 1665
Illegal XbaI site found at 944
Illegal XbaI site found at 1842
Illegal SpeI site found at 2810
Illegal PstI site found at 2819 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 935
Illegal EcoRI site found at 1665
Illegal SpeI site found at 2810
Illegal PstI site found at 2819
Illegal NotI site found at 923
Illegal NotI site found at 2829 - 21INCOMPATIBLE WITH RFC[21]Illegal suffix found in sequence at 2412
Illegal EcoRI site found at 935
Illegal EcoRI site found at 1665
Illegal BglII site found at 1280
Illegal BglII site found at 2283 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 935
Illegal EcoRI site found at 1665
Illegal XbaI site found at 944
Illegal XbaI site found at 1842
Illegal SpeI site found at 2810
Illegal PstI site found at 2819 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 935
Illegal EcoRI site found at 1665
Illegal XbaI site found at 944
Illegal XbaI site found at 1842
Illegal SpeI site found at 2810
Illegal PstI site found at 2819
Illegal NgoMIV site found at 2767 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of 500bp preceding the ORF of YCF1 was retrieved from the yeast genome database. No modifications were applied.
Source
Promoter of the vacuolar glutathione S-conjugate transporter (YDR135C) from the genome of S. Cerevisiae.