Difference between revisions of "Part:BBa K3600002"

(→‎References)
(→‎References)
Line 35: Line 35:
  
 
<HTML><a name=Lee2015 style="color:black">
 
<HTML><a name=Lee2015 style="color:black">
−
[3] Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).
+
[2] Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).
 
</a></html>
 
</a></html>

Revision as of 18:16, 14 June 2020


YCF1 promoter part plasmid

This part contains the promoter of the gene YCF1 from S. Cerevisiae.

Usage and Biology

  • activator: Yap1p
  • YCF1 gene encodes ...
  • The promoter YCF1 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to oxidative stress.
  • The binding site for Yap1p, in the promoter YCF1, is TTACTAA.1
  • The yeast toolkit for modular assembly 2 was used to assemble transcriptional units. This promoter was used as a part of type II.
  • The induction of gene transcription is mediated by stress response elements STRE.3


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
    Illegal NotI site found at 923
    Illegal NotI site found at 2829
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2412
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal BglII site found at 1280
    Illegal BglII site found at 2283
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
    Illegal NgoMIV site found at 2767
  • 1000
    COMPATIBLE WITH RFC[1000]


Measurements

References

[1]

[2] Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).