Difference between revisions of "Part:BBa K3600001"
(→Usage and Biology) |
(→References) |
||
| Line 26: | Line 26: | ||
<HTML><a name=Kuge1994 style="color:black"> | <HTML><a name=Kuge1994 style="color:black"> | ||
[1] Kuge, S. & Jones, N. YAP1 dependent activation of TRX2 is essential for the response of Saccharomyces cerevisiae to oxidative stress by hydroperoxides. The EMBO Journal 13, 655–664 (1994). | [1] Kuge, S. & Jones, N. YAP1 dependent activation of TRX2 is essential for the response of Saccharomyces cerevisiae to oxidative stress by hydroperoxides. The EMBO Journal 13, 655–664 (1994). | ||
| + | </a></html> | ||
| + | |||
| + | <HTML><a name=Martínez-Pastor1996 style="color:black"> | ||
| + | [2] Martínez-Pastor, M. T. et al. The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). The EMBO Journal 15, 2227–2235 (1996). | ||
</a></html> | </a></html> | ||
Revision as of 18:04, 14 June 2020
TRX2 promoter part plasmid
This part contains the promoter of the gene TRX2 from S. Cerevisiae.
Usage and Biology
- activator: Yap1p
- TXR2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.1
- The promoter TXR2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to oxidative stress.
- The binding site for Yap1p, in the promoter TXR2, is TTACTAA.1
- The yeast toolkit for modular assembly 2 was used to assemble transcriptional units. This promoter was used as a part of type II.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 568
Illegal BsaI.rc site found at 1089