Difference between revisions of "Part:BBa K3600000"

(→‎Usage and Biology)
(→‎References)
Line 36: Line 36:
 
<HTML><a name=Martínez-Pastor1996 style="color:black">
 
<HTML><a name=Martínez-Pastor1996 style="color:black">
 
[2] Martínez-Pastor, M. T. et al. The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). The EMBO Journal 15, 2227–2235 (1996).
 
[2] Martínez-Pastor, M. T. et al. The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). The EMBO Journal 15, 2227–2235 (1996).
 +
</a></html>
 +
 +
<HTML><a name=Lee2015 style="color:black">
 +
[3] Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).
 
</a></html>
 
</a></html>

Revision as of 17:58, 14 June 2020


HSP12 promoter part plasmid

This part contains the promoter of HSP12, a heat-shock protein in Saccharomyces Cerevisiae that is activated by the high-osmolarity glycerol (HOG) pathway.1

Usage and Biology

  • activators: MSN2/4
  • HSP12 gene encodes Saccharomyces Cerevisiae's small heat shock proteins.1
  • The promoter HSP12 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to a stress.
  • The yeast toolkit for modular assembly (ref) was used to assemble transcriptional units. This promoter was used as a part of type II.
  • The induction of gene transcription is mediated by stress response elements STRE.2


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 308
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 326
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 308
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 308
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 57
    Illegal BsaI.rc site found at 578


Measurements

References

[1] Varela, J. C., Praekelt, U. M., Meacock, P. A., Planta, R. J. & Mager, W. H. The Saccharomyces cerevisiae HSP12 gene is activated by the high-osmolarity glycerol pathway and negatively regulated by protein kinase A. Molecular and Cellular Biology 15, 6232–6245 (1995).

[2] Martínez-Pastor, M. T. et al. The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). The EMBO Journal 15, 2227–2235 (1996).

[3] Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).