Difference between revisions of "Part:BBa K3600002"
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*TXR2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.<html><a href="#Kuge1994"><sup>1</sup></a></html>
 
*TXR2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.<html><a href="#Kuge1994"><sup>1</sup></a></html>
 
*The promoter TXR2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to an oxidative stress.
 
*The promoter TXR2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to an oxidative stress.
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<!-- -->*The binding site for Yap1p, in the promoter YCF1, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a><!-- --></html>
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*The binding site for Yap1p, in the promoter YCF1, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a>
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Revision as of 17:53, 14 June 2020


YCF1 promoter part plasmid

This part contains the promoter of the gene TRX2 from S. Cerevisiae.

Usage and Biology

  • activator: Yap1p
  • TXR2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.1
  • The promoter TXR2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to an oxidative stress.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
    Illegal NotI site found at 923
    Illegal NotI site found at 2829
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2412
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal BglII site found at 1280
    Illegal BglII site found at 2283
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
    Illegal NgoMIV site found at 2767
  • 1000
    COMPATIBLE WITH RFC[1000]


Measurements

References

[1] Kuge, S. & Jones, N. YAP1 dependent activation of TRX2 is essential for the response of Saccharomyces cerevisiae to oxidative stress by hydroperoxides. The EMBO Journal 13, 655–664 (1994).