Difference between revisions of "Part:BBa K3600002"

 
Line 5: Line 5:
 
.
 
.
  
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
* activator: Yap1p
 +
*TXR2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.<html><a href="#Kuge1994"><sup>1</sup></a></html>
 +
*The promoter TXR2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to an oxidative stress.
 +
<!-- -->*The binding site for Yap1p, in the promoter YCF1, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a><!-- --></html>
 +
  
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3600002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3600002 SequenceAndFeatures</partinfo>

Revision as of 17:45, 14 June 2020


YCF1 promoter part plasmid

.

Usage and Biology

  • activator: Yap1p
  • TXR2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.1
  • The promoter TXR2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to an oxidative stress.
  • The binding site for Yap1p, in the promoter YCF1, is TTACTAA.1


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
    Illegal NotI site found at 923
    Illegal NotI site found at 2829
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2412
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal BglII site found at 1280
    Illegal BglII site found at 2283
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
    Illegal NgoMIV site found at 2767
  • 1000
    COMPATIBLE WITH RFC[1000]