Difference between revisions of "Part:BBa K3515000:Design"
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===References=== | ===References=== | ||
| + | Chao, H., Bautista, D.L., Litowski, J., Irvin, R.T. and Hodges, R.S., 1998. Use of a heterodimeric coiled-coil system for biosensor application and affinity purification. Journal of Chromatography B: Biomedical Sciences and Applications, 715(1), pp.307-329. | ||
Latest revision as of 14:20, 25 May 2020
E coil alpha helix with cysteine to be used with a K coil alpha helix for protein immobilization.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added a cysteine for immobilization (C31). Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.
Source
The source of this part is its five-heptad repeat designed by Chao et al. (https://doi.org/10.1016/S0378-4347(98)00172-8).
References
Chao, H., Bautista, D.L., Litowski, J., Irvin, R.T. and Hodges, R.S., 1998. Use of a heterodimeric coiled-coil system for biosensor application and affinity purification. Journal of Chromatography B: Biomedical Sciences and Applications, 715(1), pp.307-329.