Difference between revisions of "Part:BBa K143069:Design"

 
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<partinfo>BBa_K143069 short</partinfo>
 
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<partinfo>BBa_K143069 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143069 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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The amyE 5' integration sequence was PCR cloned from the ''B. subtilis'' integration vector pDR111 utilising Pfu DNA polymerase. The sequence of Pveg-gsiB was obtained from papers.  
 
The amyE 5' integration sequence was PCR cloned from the ''B. subtilis'' integration vector pDR111 utilising Pfu DNA polymerase. The sequence of Pveg-gsiB was obtained from papers.  
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The amyE 5' integration sequence was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the Pveg-gsiB that was synthesised by GeneArt
 
The amyE 5' integration sequence was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the Pveg-gsiB that was synthesised by GeneArt
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===References===
 

Revision as of 16:28, 27 October 2008

AmyE integratable PoPS generator (Pveg-gsiB)



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of Pveg-gsiB was obtained from papers.


Source

The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the Pveg-gsiB that was synthesised by GeneArt