Difference between revisions of "Part:BBa K3515009:Design"

 
Line 16: Line 16:
  
 
===References===
 
===References===
 +
Fehr, M., Lalonde, S., Lager, I., Wolff, M.W. and Frommer, W.B., 2003. In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7 cells by fluorescent nanosensors. Journal of Biological Chemistry, 278(21), pp.19127-19133.

Revision as of 13:49, 25 May 2020


Glucose binding protein with cysteine modification(s) to bind to a biosensor and FRET to monitor glu


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added a cysteine for immobilization (D190C) opposite to the active site region for linker arm immobilization. Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.


Source

The source of this part is its sequence retrieved from the European Nucleotide Archive (M59444.1) along with our own modifications.

References

Fehr, M., Lalonde, S., Lager, I., Wolff, M.W. and Frommer, W.B., 2003. In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7 cells by fluorescent nanosensors. Journal of Biological Chemistry, 278(21), pp.19127-19133.