Difference between revisions of "Part:BBa K3515005:Design"
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===References=== | ===References=== | ||
+ | Fehr, M., Lalonde, S., Lager, I., Wolff, M.W. and Frommer, W.B., 2003. In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7 cells by fluorescent nanosensors. Journal of Biological Chemistry, 278(21), pp.19127-19133. |
Latest revision as of 18:24, 22 May 2020
Glucose binding protein with cysteine modification(s) to bind to a biosensor.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added a cysteine for immobilization (D190C) opposite to the active site region for linker arm immobilization. Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.
Source
The source of this part is its sequence retrieved from the European Nucleotide Archive (M59444.1) along with our own modifications.
References
Fehr, M., Lalonde, S., Lager, I., Wolff, M.W. and Frommer, W.B., 2003. In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7 cells by fluorescent nanosensors. Journal of Biological Chemistry, 278(21), pp.19127-19133.