Difference between revisions of "Part:BBa K2984033:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Secretion vector for C.reinhardtii designed for Transformation in Clamydomnasd and further cloning into Level 2 backbone with MoClo Standard
+
Secretion vector designed for transformation in <i>C. reinhardtii</i> and further cloning into a level 2 backbone (according to the MoClo standard) by GoldenGate cloning.
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
The construct consist of the four different Level 0 parts AR (BBa_K2984025),ARS (BBa_K2984000), YFP (BBa_K2984017) and Rbcs2 (BBa_K2984018).
+
The construct consists of the four different Level 0 parts AR (BBa_K2984025), ARS (BBa_K2984000), YFP (BBa_K2984017) and RbcS2 (BBa_K2984018).
−
which were cloned into the level 1c Backbone (BBa_K2984002) using the MoClo Standard.
+
which were cloned into the level 1c backbone (BBa_K2984002) using the MoClo Standard.
  
 
===References===
 
===References===
 +
 +
Schroda, M., Blöcker, D., & Beck, C. F. (2000). The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas. The plant journal, 21(2), 121-131.
 +
 +
N. J. Patron, D. Orzaez, et al. „Standards for plant synthetic biology: a
 +
common syntax for exchange of DNA parts“. In: New Phytologist 208.1 (Oct.
 +
2015), pp. 13–19. issn: 0028-646X.

Latest revision as of 13:44, 7 January 2020


L1c-AR-ARS-YFP-Rbcs2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 953
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 268
    Illegal PstI site found at 953
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 953
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 953
    Illegal NgoMIV site found at 1430
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Secretion vector designed for transformation in C. reinhardtii and further cloning into a level 2 backbone (according to the MoClo standard) by GoldenGate cloning.


Source

The construct consists of the four different Level 0 parts AR (BBa_K2984025), ARS (BBa_K2984000), YFP (BBa_K2984017) and RbcS2 (BBa_K2984018). which were cloned into the level 1c backbone (BBa_K2984002) using the MoClo Standard.

References

Schroda, M., Blöcker, D., & Beck, C. F. (2000). The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas. The plant journal, 21(2), 121-131.

N. J. Patron, D. Orzaez, et al. „Standards for plant synthetic biology: a common syntax for exchange of DNA parts“. In: New Phytologist 208.1 (Oct. 2015), pp. 13–19. issn: 0028-646X.