Difference between revisions of "Part:BBa K117004"

 
m
 
(4 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
+
<html>
 +
<div id="arrow">
 +
<a href="http://2008.igem.org/Team:NTU-Singapore">
 +
<img src="https://static.igem.org/mediawiki/parts/2/26/Back_to_ntu_copy.png"
 +
    alt="Back to NTU@iGEM"
 +
    title="Back to NTU@iGEM">
 +
</a>
 +
</div>
 +
</html>
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K117004 short</partinfo>
+
<partinfo>BBa_K117004 short</partinfo> <br>
 +
 
 +
='''The construct design'''=
 +
[[Image:NTU@iGEM_PLacI_GFP_pic.JPG|thumb|center|400px| ‎BBa K117004 ]]
  
−
This composite part expresses GFP when being induced by lactose.
+
This biobrick device has a lacI regulated promoter which upon the induction of lactose/IPTG, will casue the expression of the reporter gene, Green fluorescence protein (GFP EE0040).
 +
[[Image:NTU LacI GFP.png|thumb|center|800px| ''‎Function of K117004'' ]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:58, 27 October 2008

pLacI-GFP

The construct design

‎BBa K117004

This biobrick device has a lacI regulated promoter which upon the induction of lactose/IPTG, will casue the expression of the reporter gene, Green fluorescence protein (GFP EE0040).

‎Function of K117004

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 873