Difference between revisions of "Part:BBa K2984030:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The construct was designed to test secretion by <i>C. reinhardtii<i> and to be used for Level 2 MoClo Ligation
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The construct was designed to test secretion by <i>C. reinhardtii</i> and to be used for Level 2 MoClo Ligation
  
 
===Source===
 
===Source===

Latest revision as of 14:24, 26 November 2019


L1c-Psad-ARS-YFP-SP20-Rbcs2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1110
    Illegal NgoMIV site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construct was designed to test secretion by C. reinhardtii and to be used for Level 2 MoClo Ligation

Source

The construct was created through a Level-1 ligation following the the MoClo Standard (based on Golden Gate assembly) and contains these 5 different Level-0 parts: PsaD (BBa_K2984008), ARS (BBa_K2984000), YFP (BBa_K2984017), SP20 (BBa_K2984016) and RbcS2 (BBa_K2984018).

References