Difference between revisions of "Part:BBa K3122006"
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Latest revision as of 09:44, 22 November 2019
Inducible Ptac promoter (TypeIIS adapted)
Ptac, trp and lac regulated promoter (Part: BBa_K864400), adapted to MoClo standard by our team. It was designed to control Streptavidin-CBDs expression via IPTG induction.
Description
Promotor Ptac is a combination of the different promoters from the lac and tpr operons. The advantages of this promoter include its high efficiency (11 times more than the lac one and 3 times more than the tpr one) and the fine control of the expression it allows (it can be repressed by lac repressor and derepressed with isopropyl-beta-D-thiogalactoside).
Characterization
Characterization of this part was planned to be conducted by building three transcriptional units ( BBa_K3122016, BBa_K3122017, BBa_K3122018) expressing different recombinant proteins. Unfortunately, no results were obtained.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
de Boer, H., Comstock, L. and Vasser, M. (1983). The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences, 80(1), pp.21-25.