Difference between revisions of "Part:BBa K3304016"
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<partinfo>BBa_K3304016 short</partinfo> | <partinfo>BBa_K3304016 short</partinfo> | ||
− | Amil cp used | + | Amil cp used as a reporter for colony picking. we modified the standard composite functional AmilCP part with primers that insert BsaI recognition sites so that we can furthermore insert the gates into bacterial plasmids PSB1C3 |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 03:59, 22 October 2019
Amil Cp with BsaI recognition sites that insert PvuII
Amil cp used as a reporter for colony picking. we modified the standard composite functional AmilCP part with primers that insert BsaI recognition sites so that we can furthermore insert the gates into bacterial plasmids PSB1C3
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 16
Illegal NheI site found at 39 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 740
Illegal BsaI.rc site found at 4