Difference between revisions of "Part:BBa K3091000"
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<p>We built this new part as an approach to detecting bacteriophages in fecal polluted water through color change. This enzyme is from Solanum tuberosum, and would cause yellow solution containing tea polyphenol turn red. Also, the product of the reaction, theaflavins, has multiple health benefits. Our bacteria can potentially also play a part in theaflavins industrial production.</p> | <p>We built this new part as an approach to detecting bacteriophages in fecal polluted water through color change. This enzyme is from Solanum tuberosum, and would cause yellow solution containing tea polyphenol turn red. Also, the product of the reaction, theaflavins, has multiple health benefits. Our bacteria can potentially also play a part in theaflavins industrial production.</p> | ||
| − | <h1>Plasmid Construction</ | + | <h1>Plasmid Construction</h1> |
<p>We acquired the gene sequence through NCBI blast, and synthesized the sequence thanks to Twist, and selected pET expression system due to its high efficiency and capability to be regulated by IPTG induction.</p> | <p>We acquired the gene sequence through NCBI blast, and synthesized the sequence thanks to Twist, and selected pET expression system due to its high efficiency and capability to be regulated by IPTG induction.</p> | ||
Latest revision as of 03:47, 22 October 2019
Polyphenol Oxidase [PPO]
Polyphenol oxidase is the biocatalyst of tea polyphenol and is able to catalyze redox reactions to form quinone as final product. The reaction is chromogenic and is widely observed in plants such as apple, litchi, potato and spinach. Part BBa_K3091000's sequence comes from PPO found in potato.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1180
Illegal PstI site found at 434 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1180
Illegal PstI site found at 434 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1180
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1180
Illegal PstI site found at 434 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1180
Illegal PstI site found at 434
Illegal AgeI site found at 1009 - 1000COMPATIBLE WITH RFC[1000]