Difference between revisions of "Part:BBa K2966006"
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<h2>Characterization</h2>
 
<h2>Characterization</h2>
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The figure below shows Pc with NodC_Pf, a negative control, and PpsbA with NodC_Pf. The negative control was used to show that the wildtype E. coli does not normally fluoresce. Pc with NodC_Pf had almost all of its cells fluoresce blue, and PpsbA with NodC_Pf had most of its cells fluoresce as well.
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https://2019.igem.org/wiki/images/b/b8/T--Purdue--S3controlS4.jpg
 
https://2019.igem.org/wiki/images/b/b8/T--Purdue--S3controlS4.jpg
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The figure above shows Pc with NodC_Pf, a negative control, and PpsbA with NodC_Pf. The negative control was used to show that the wildtype E. coli does not normally fluoresce. Pc with NodC_Pf had almost all of its cells fluoresce blue, and PpsbA with NodC_Pf had most of its cells fluoresce as well.
 
  
  

Revision as of 03:39, 22 October 2019


PpsbA-NodC_Pf-T4 term

This part is the constitutive promoter, Pc along with the codon-optimized NodC for P. fluorescens and finally the T4 transcriptional terminator.

Characterization

The figure below shows Pc with NodC_Pf, a negative control, and PpsbA with NodC_Pf. The negative control was used to show that the wildtype E. coli does not normally fluoresce. Pc with NodC_Pf had almost all of its cells fluoresce blue, and PpsbA with NodC_Pf had most of its cells fluoresce as well.

T--Purdue--S3controlS4.jpg


50px Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]