Difference between revisions of "Part:BBa K3189015"

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<b>Figure 1: E. coli BL21(DE3) BBa_K3189015 transformants from 11 different colonies grown in LB+tetracycline.</b> The three wells in the top right hand corner are negative controls (E. coli without BBa_K3189015). A dark colour of variable intensity is visible at 100 ng/mL tetracycline for all of the samples.
 
<b>Figure 1: E. coli BL21(DE3) BBa_K3189015 transformants from 11 different colonies grown in LB+tetracycline.</b> The three wells in the top right hand corner are negative controls (E. coli without BBa_K3189015). A dark colour of variable intensity is visible at 100 ng/mL tetracycline for all of the samples.
 
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The construct [[BBa_K3189015]] containing the chromoprotein amilCP ([[BBa_K1343022]]) under the control of [[BBa_K3189001]]. When the system is induced with 100 ng/mL of tetracycline, a dark blue colour is produced (Figure 4 and Figure 5). This shows [[BBa_K3189001]] is able to function with different reporter proteins other than just <i>gfp</i>.
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The construct [[BBa_K3189015]] containing the chromoprotein amilCP ([[BBa_K1343022]]) under the control of [[BBa_K3189001]]. When the system is induced with 100 ng/mL of tetracycline, a dark blue colour is produced (Figure 2 and Figure 3). This shows [[BBa_K3189001]] is able to function with different reporter proteins other than just <i>gfp</i>.
 
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https://static.igem.org/mediawiki/parts/thumb/f/f5/T--Guelph--pTA-tetnotet.jpg/216px-T--Guelph--pTA-tetnotet.jpg
 
https://static.igem.org/mediawiki/parts/thumb/f/f5/T--Guelph--pTA-tetnotet.jpg/216px-T--Guelph--pTA-tetnotet.jpg
 
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<b>Figure 4: [[BBa_K3189015]] containing cells in LB broth.</b> amilCP expression being induced using 100 ng/mL tetracycline (left) and uninduced (right).
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<b>Figure 2: [[BBa_K3189015]] containing cells in LB broth.</b> amilCP expression being induced using 100 ng/mL tetracycline (left) and uninduced (right).
 
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https://static.igem.org/mediawiki/parts/thumb/2/29/T--Guelph--pTAtetnotetspun.jpg/207px-T--Guelph--pTAtetnotetspun.jpg
 
https://static.igem.org/mediawiki/parts/thumb/2/29/T--Guelph--pTAtetnotetspun.jpg/207px-T--Guelph--pTAtetnotetspun.jpg
 
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<b>Figure 5: Pellets of cells of [[BBa_K3189015]] induced and uninduced with tetracycline.</b> Pellet of cells induced with 100 ng/mL tetracycline (left) and pellet of cells uninduced (right).
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<b>Figure 3: Pellets of cells of [[BBa_K3189015]] induced and uninduced with tetracycline.</b> Pellet of cells induced with 100 ng/mL tetracycline (left) and pellet of cells uninduced (right).
 
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Revision as of 03:37, 22 October 2019


Tet Controlled amilCP

Tet Controlled amilCP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1758
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 146
    Illegal XhoI site found at 139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 80


test
T--Guelph--BBa_3189015a.png
Figure 1: E. coli BL21(DE3) BBa_K3189015 transformants from 11 different colonies grown in LB+tetracycline. The three wells in the top right hand corner are negative controls (E. coli without BBa_K3189015). A dark colour of variable intensity is visible at 100 ng/mL tetracycline for all of the samples.

The construct BBa_K3189015 containing the chromoprotein amilCP (BBa_K1343022) under the control of BBa_K3189001. When the system is induced with 100 ng/mL of tetracycline, a dark blue colour is produced (Figure 2 and Figure 3). This shows BBa_K3189001 is able to function with different reporter proteins other than just gfp.

216px-T--Guelph--pTA-tetnotet.jpg
Figure 2: BBa_K3189015 containing cells in LB broth. amilCP expression being induced using 100 ng/mL tetracycline (left) and uninduced (right).

207px-T--Guelph--pTAtetnotetspun.jpg
Figure 3: Pellets of cells of BBa_K3189015 induced and uninduced with tetracycline. Pellet of cells induced with 100 ng/mL tetracycline (left) and pellet of cells uninduced (right).