Difference between revisions of "Part:BBa K3189015"
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− | <b>Figure 4: [[ | + | <b>Figure 4: [[BBa_K3189015]] containing cells in LB broth.</b> amilCP expression being induced using 100 ng/mL tetracycline (left) and uninduced (right). |
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− | <b>Figure 5: Pellets of cells of [[ | + | <b>Figure 5: Pellets of cells of [[BBa_K3189015]] induced and uninduced with tetracycline.</b> Pellet of cells induced with 100 ng/mL tetracycline (left) and pellet of cells uninduced (right). |
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Revision as of 03:36, 22 October 2019
Tet Controlled amilCP
Tet Controlled amilCP
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1758
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 146
Illegal XhoI site found at 139 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 80
test
Figure 1: E. coli BL21(DE3) BBa_K3189015 transformants from 11 different colonies grown in LB+tetracycline. The three wells in the top right hand corner are negative controls (E. coli without BBa_K3189015). A dark colour of variable intensity is visible at 100 ng/mL tetracycline for all of the samples.
The construct BBa_K3189015 containing the chromoprotein amilCP (BBa_K1343022) under the control of BBa_K3189001. When the system is induced with 100 ng/mL of tetracycline, a dark blue colour is produced (Figure 4 and Figure 5). This shows BBa_K3189001 is able to function with different reporter proteins other than just gfp.
Figure 4: BBa_K3189015 containing cells in LB broth. amilCP expression being induced using 100 ng/mL tetracycline (left) and uninduced (right).
Figure 5: Pellets of cells of BBa_K3189015 induced and uninduced with tetracycline. Pellet of cells induced with 100 ng/mL tetracycline (left) and pellet of cells uninduced (right).