Difference between revisions of "Part:BBa K2916013"

 
 
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Aspartate-tRNA synthetase is a fusion protein that is used in the PURE and OnePot PURE cell-free systems.
 
  
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Inorganic pyrophosphatase is a protein that is used in the PURE and OnePot PURE cell-free systems.
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===Usage and Biology===
 
===Usage and Biology===
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Inorganic pyrophosphatase (PPiase) is responsible for regulating the secondary structure of the expressed proteins.
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In our part, besides the sequence encoding for the protein we also have a hexahistidine-tag to allow us purify the protein.
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In our project we used GlnRS as a part of the protein solution needed for <html><a style="padding: 0px; margin: 0px;" href="https://2019.igem.org/Team:EPFL/OnePot_Pure"> OnePot PURE cell-free system </a></html> produced with the method of gravity flow affinity chromatography, as described in the <html><a style="padding: 0px; margin: 0px;" href="https://www.protocols.io/view/protein-purification-for-onepot-pure-cell-free-sys-8auhsew"> protocol </a></html> we designed.
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===Characterization===
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All the data regarding the characterization of that part can be found in the composite part: <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K2916048"> BBa_K2916048 </a></html>.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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Latest revision as of 03:34, 22 October 2019


PPiase protein equipped with a 6x HIS affinity tag


Inorganic pyrophosphatase is a protein that is used in the PURE and OnePot PURE cell-free systems.

Usage and Biology

Inorganic pyrophosphatase (PPiase) is responsible for regulating the secondary structure of the expressed proteins.

In our part, besides the sequence encoding for the protein we also have a hexahistidine-tag to allow us purify the protein.


In our project we used GlnRS as a part of the protein solution needed for OnePot PURE cell-free system produced with the method of gravity flow affinity chromatography, as described in the protocol we designed.


Characterization

All the data regarding the characterization of that part can be found in the composite part: BBa_K2916048 .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 862
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 451