Difference between revisions of "Part:BBa K3033012"

Latest revision as of 03:31, 22 October 2019

NSP4

Derived from E.coli and synthesized through IDT.

A transmembrane glycoprotein, NSP4 is organized into three main domains: a three-helical TM domain in the N-terminus (also a viroporin domain), a central cytoplasmic coiled-coil domain for multimerization, and an C-terminal flexible region. It can also be secreted out of the cell. It codes for a secretion signal sequence which would help our tyrosinase enzyme to be transported into the medium.

Usage and Biology

Team can use this signal sequence in order to secrete their protein into the supernatant

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 48
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3033012 short</partinfo>
-A transmembrane glycoprotein, NSP4 is organized into three main domains: a three-helical TM domain in the N-terminus (also a viroporin domain), a central cytoplasmic coiled-coil domain for multimerization, and an C-terminal flexible region. It can also be secreted out of the cell. It  codes for a secretion signal sequence which would help our tyrosinase enzyme to be transported into the medium
+Derived from E.coli and synthesized through IDT.
-Derived from E.coli and synthesized through IDT. This codes for a secretion signal sequence which would help our tyrosinase enzyme to be transported into the medium
+A transmembrane glycoprotein, NSP4 is organized into three main domains: a three-helical TM domain in the N-terminus (also a viroporin domain), a central cytoplasmic coiled-coil domain for multimerization, and an C-terminal flexible region. It can also be secreted out of the cell. It  codes for a secretion signal sequence which would help our tyrosinase enzyme to be transported into the medium.
 ===Usage and Biology===