Difference between revisions of "Part:BBa K3033012"
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<partinfo>BBa_K3033012 short</partinfo> | <partinfo>BBa_K3033012 short</partinfo> | ||
| − | Derived from E.coli and synthesized through IDT. | + | Derived from E.coli and synthesized through IDT. |
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| + | A transmembrane glycoprotein, NSP4 is organized into three main domains: a three-helical TM domain in the N-terminus (also a viroporin domain), a central cytoplasmic coiled-coil domain for multimerization, and an C-terminal flexible region. It can also be secreted out of the cell. It codes for a secretion signal sequence which would help our tyrosinase enzyme to be transported into the medium. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 03:31, 22 October 2019
NSP4
Derived from E.coli and synthesized through IDT.
A transmembrane glycoprotein, NSP4 is organized into three main domains: a three-helical TM domain in the N-terminus (also a viroporin domain), a central cytoplasmic coiled-coil domain for multimerization, and an C-terminal flexible region. It can also be secreted out of the cell. It codes for a secretion signal sequence which would help our tyrosinase enzyme to be transported into the medium.
Usage and Biology
Team can use this signal sequence in order to secrete their protein into the supernatant
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 48
- 1000COMPATIBLE WITH RFC[1000]