Difference between revisions of "Part:BBa K2916012:Design"

 
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K2916011 short</partinfo>
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<partinfo>BBa_K2916012 short</partinfo>
  
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<partinfo>BBa_K2916011 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2916012 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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none
 
  
 +
The sequence was extracted from the pQE30-His-NDK plasmid and expressed in the M15 E.coli strain.
 +
 +
Codon optimization : 135 from G to C
 +
 +
Codon optimization : 243 from T to C
 +
 +
Codon optimization : 486 from C to G
 +
 +
Second stop codon was added in compliance with iGEM standards.
  
  
 
===Source===
 
===Source===
  
−
SnapGene
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Sebastian Maerkl Lab at EPFL, Switzerland.
 +
 
 +
http://www.addgene.org/124136/
  
 
===References===
 
===References===
 +
<I>
 +
Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568
 +
 +
Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.
 +
</I>

Latest revision as of 03:30, 22 October 2019


NDK protein eqquiped with a 6x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 31
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was extracted from the pQE30-His-NDK plasmid and expressed in the M15 E.coli strain.

Codon optimization : 135 from G to C

Codon optimization : 243 from T to C

Codon optimization : 486 from C to G

Second stop codon was added in compliance with iGEM standards.


Source

Sebastian Maerkl Lab at EPFL, Switzerland.

http://www.addgene.org/124136/

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568

Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.