Difference between revisions of "Part:BBa K3033006"
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<partinfo>BBa_K3033006 short</partinfo> | <partinfo>BBa_K3033006 short</partinfo> | ||
| − | Synthesized through IDT. This tag was used for protein detection through western blotting and protein purification | + | Synthesized through IDT. This tag was used for protein detection through western blotting and protein purification. The His-tag (also called 6xHis-tag) is one of the simplest and most widely used purification tags, with six or more consecutive histidine residues. These residues readily coordinate with transition metal ions such as Ni2+ or Co2+ immobilized on beads or a resin for purification. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 03:27, 22 October 2019
6-His Tag
Synthesized through IDT. This tag was used for protein detection through western blotting and protein purification. The His-tag (also called 6xHis-tag) is one of the simplest and most widely used purification tags, with six or more consecutive histidine residues. These residues readily coordinate with transition metal ions such as Ni2+ or Co2+ immobilized on beads or a resin for purification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]