Difference between revisions of "Part:BBa K2926006"

Latest revision as of 03:22, 22 October 2019

SUP4 yeast terminator

Usage and Biology

SUP4 is a natural terminator of Saccharomyces cerevisiae, belonging to the SUP4 tRNA^Tyr locus.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Plasmid Design

For the analysis and characterization of the SNR52 Promotor in combiantion with the SUP4 Terminaror, mCherry was cloned beteen them using Gibson Assembly. The SNR52 Promotor and SUP4 Terminator where optained as a gBlock from IDT.

Sequencing Results

The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.

Characterisation

We created a new protocol for the calibration of mCherry measurements with TexasRed, based on the standard iGEM protocoll for GFP fluorescence calibration, We performed the analysis with E. coli DH5α as well as S. cerevisiae cultures.
See here for the characterization results.

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-<span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K2926006 SequenceAndFeatures</partinfo>
-__TOC__
-<!-- Uncomment this to enable Functional Parameter display
-===Functional Parameters===
-<partinfo>BBa_K2926003 parameters</partinfo>
-<!-- -->
-==Characterisation==
-<html>
-<style>
-.table, .table th, .table td{
-	border-collapse: collapse;
-	border: 1px solid #dddddd;
-}
-.table {
-	width: 96%;
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-	margin: auto;
-	margin-top: 15x;
-	margin-bottom: 15px;
-}
-.table th{
-	background-color: #efefef;
-	text-align: center;
-}
-.table tr {
-	background-color: #ffffff;
-	height: 30px;
-}
-.table td  {
-	padding: 10px;
-}
-.table tr:hover {
-	background-color: #efefef;
-}
-</style>
-</html>
-__NOTOC__
-<partinfo>BBa_K2926005 short</partinfo>
-==Usage and Biology==
-<html>
-<p>
-SUP4 is a bla bla bla bla bla
-</p>
-</html>
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K2926005 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K2926006 SequenceAndFeatures</partinfo>
 __TOC__
@@ Line 120: / Line 62: @@
 ==Plasmid Design==
 <html>
-For the analysis and characterization of the SNR52 Promotor <a href="XXX">XXXX</a>
+For the analysis and characterization of the SNR52 Promotor
-in combiantion with the SUP4 Terminaror <a href="XXX">XXXX</a>,
+in combiantion with the SUP4 Terminaror,
-mCherry <a href="XXX">XXXX</a> was cloned beteen them using Gibson Assembly.
+mCherry was cloned beteen them using Gibson Assembly.
 The SNR52 Promotor and SUP4 Terminator where optained as a gBlock from IDT.
 </html>
@@ Line 139: / Line 81: @@
 We performed the analysis with <i>E. coli DH5α</i> as well as <i>S. cerevisiae</i> cultures.
 <br>
-See <a href="XXX">BBa_K2926072</a> and <a href="XXX">BBa_K2926073</a> for the characterization results.
+See <a href="https://parts.igem.org/Part:BBa_K2926003">here</a> for the characterization results.
 </html>