Difference between revisions of "Part:BBa K2926006"

 
 
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__NOTOC__
 
__NOTOC__
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{|width='80%'
 
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|-
 
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|width=35% valign='top'|
 
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[[Image:Terminator.png]]
 
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<partinfo>BBa_K2926006 parameters</partinfo>
 
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| width=50% valign='top' style='border: 1px solid black'|
 
 
<partinfo>BBa_K2926006 short</partinfo>
 
<partinfo>BBa_K2926006 short</partinfo>
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* description
 
  
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|}
 
  
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'''Secondary Structure'''
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==Usage and Biology==
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<p>
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SUP4 is a natural terminator of Saccharomyces cerevisiae, belonging to the SUP4 tRNA<sup>Tyr</sup> locus.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2926006 SequenceAndFeatures</partinfo>
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__TOC__
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2926003 parameters</partinfo>
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<!-- -->
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==Plasmid Design==
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For the analysis and characterization of the SNR52 Promotor
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in combiantion with the SUP4 Terminaror,
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mCherry was cloned beteen them using Gibson Assembly.
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The SNR52 Promotor and SUP4 Terminator where optained as a gBlock from IDT.
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===Sequencing Results===
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The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.
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==Characterisation==
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We created a new <a href="https://2019.igem.org/Team:Bielefeld-CeBiTec/mCherry">protocol for the calibration of mCherry</a> measurements with TexasRed,
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based on the standard <a href="https://www.protocols.io/view/calibration-protocol-plate-reader-fluorescence-cal-6zrhf56">iGEM protocoll for GFP fluorescence calibration</a>,
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We performed the analysis with <i>E. coli DH5α</i> as well as <i>S. cerevisiae</i> cultures.
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<br>
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See <a href="https://parts.igem.org/Part:BBa_K2926003">here</a> for the characterization results.
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[[Image:Mfold-K2926006-1.png]]
 
  
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<hr>
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==References==
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'''Measurement'''
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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Latest revision as of 03:22, 22 October 2019


SUP4 yeast terminator


Usage and Biology

SUP4 is a natural terminator of Saccharomyces cerevisiae, belonging to the SUP4 tRNATyr locus.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Design

For the analysis and characterization of the SNR52 Promotor in combiantion with the SUP4 Terminaror, mCherry was cloned beteen them using Gibson Assembly. The SNR52 Promotor and SUP4 Terminator where optained as a gBlock from IDT.


Sequencing Results

The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.


Characterisation

We created a new protocol for the calibration of mCherry measurements with TexasRed, based on the standard iGEM protocoll for GFP fluorescence calibration, We performed the analysis with E. coli DH5α as well as S. cerevisiae cultures.
See here for the characterization results.



References