Difference between revisions of "Part:BBa K2926005"
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==Plasmid Design==
 
==Plasmid Design==
 
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For the analysis and characterization of the GALL Promotor <a href="XXX">XXXX</a>
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For the analysis and characterization of the GALL Promotor  
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in combiantion with the TPs1 Terminaror <a href="XXX">XXXX</a>,
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in combiantion with the TPs1 Terminaror,
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mCherry <a href="XXX">XXXX</a> was cloned beteen them using Gibson Assembly.
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mCherry was cloned beteen them using Gibson Assembly.
 
The GALL Promotor and TPs1 Terminator where optained as a gBlock from IDT.
 
The GALL Promotor and TPs1 Terminator where optained as a gBlock from IDT.
 
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==Characterisation==
 
==Characterisation==
 
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We followed the standard <a href="https://www.protocols.io/view/calibration-protocol-plate-reader-fluorescence-cal-6zrhf56">iGEM protocoll for GFP fluorescence calibration</a>,
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For the characteriastion of this part have a look <a href="https://parts.igem.org/Part:BBa_K2926003"><a> here
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measuring our cultures against Flourescin.
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We performed the analysis with <i>E. coli DH5α</i> as well as <i>S. cerevisiae</i> cultures.
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<br>
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See <a href="XXX">BBa_K2926073</a> for the characterization results.
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==References==
 
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Revision as of 03:19, 22 October 2019


TPs1 yeast terminator


Usage and Biology

TPs1 is the natural terminator of the tps1 gen in Saccharomyces cerevisiae, which is encoding the trehalose-6-phosphate synthetase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Design

For the analysis and characterization of the GALL Promotor in combiantion with the TPs1 Terminaror, mCherry was cloned beteen them using Gibson Assembly. The GALL Promotor and TPs1 Terminator where optained as a gBlock from IDT.


Sequencing Results

The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.

Characterisation

For the characteriastion of this part have a look here