Difference between revisions of "Part:BBa K2926005"
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==Plasmid Design== | ==Plasmid Design== | ||
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− | For the analysis and characterization of the GALL Promotor | + | For the analysis and characterization of the GALL Promotor |
− | in combiantion with the TPs1 Terminaror | + | in combiantion with the TPs1 Terminaror, |
− | mCherry | + | mCherry was cloned beteen them using Gibson Assembly. |
The GALL Promotor and TPs1 Terminator where optained as a gBlock from IDT. | The GALL Promotor and TPs1 Terminator where optained as a gBlock from IDT. | ||
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==Characterisation== | ==Characterisation== | ||
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− | + | For the characteriastion of this part have a look <a href="https://parts.igem.org/Part:BBa_K2926003"><a> here | |
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Revision as of 03:19, 22 October 2019
TPs1 yeast terminator
Usage and Biology
TPs1 is the natural terminator of the tps1 gen in Saccharomyces cerevisiae, which is encoding the trehalose-6-phosphate synthetase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Plasmid Design
For the analysis and characterization of the GALL Promotor in combiantion with the TPs1 Terminaror, mCherry was cloned beteen them using Gibson Assembly. The GALL Promotor and TPs1 Terminator where optained as a gBlock from IDT.
Sequencing Results
The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.
Characterisation
For the characteriastion of this part have a look here