Difference between revisions of "Part:BBa K2957003"
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<b>How to use it?</b>: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.
 
<b>How to use it?</b>: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.
  
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https://static.igem.org/mediawiki/parts/5/50/T--MIT----SecretionTest.png
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:10, 22 October 2019


hEF1a, IL-8 (CXCL-8)

Contents: hEF1a, IL-8 (CXCL-8), SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and IL-8 gene allowing for constitutive IL-8 expression

What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 567
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 485
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 567
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 567
    Illegal AgeI site found at 81
    Illegal AgeI site found at 573
  • 1000
    COMPATIBLE WITH RFC[1000]