Difference between revisions of "Part:BBa K2800025"

Revision as of 03:03, 22 October 2019

tetR/tetA promoter

-

Usage and Biology

We constructed this part based on the part of Ptet (BBa_K2800025). As we all know that the inducible promoter Ptet exhibits leakage expression. Even in the absence of tetracycline, expression of gene in Z. mobilis still. A ribosome binding site, or ribosomal binding site (RBS), is a sequence of nucleotides upstream of the start codon of an mRNA transcript that is responsible for the recruitment of a ribosome during the initiation of protein translation. So we can weaken the expression of the promoter Ptet by damaging of the sequence of ribosome binding. We reduced the leakage of the promoter expression by adding six bases after the sequence of the promoter Ptet, thereby reducing the influence of promoter leakage on cell metabolism.

File:T--HUBU-WUHAN--impro1.png

Fig 1. the signal of the mcherry fluorescence.

File:T--HUBU-WUHAN--impro2.png

Fig 2. the signal of the mcherry fluorescence

File:T--HUBU-WUHAN--impro3.png

Fig 3. the red fluorescence intensity and the OD 600nm of the cells

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 -
-<!-- Add more about the biology of this part here
-===Usage and Biology===
+===Usage and Biology===
+We constructed this part based on the part of Ptet (BBa_K2800025).
+As we all know that the inducible promoter Ptet exhibits leakage expression. Even in the absence of tetracycline, expression of gene in Z. mobilis still.
+A ribosome binding site, or ribosomal binding site (RBS), is a sequence of nucleotides upstream of the start codon of an mRNA transcript that is responsible for the recruitment of a ribosome during the initiation of protein translation.
+So we can weaken the expression of the promoter Ptet by damaging of the sequence of ribosome binding. We reduced the leakage  of the promoter expression by adding six bases after the sequence of the promoter Ptet, thereby reducing the influence of promoter leakage on cell metabolism.
+[[File:T--HUBU-WUHAN--impro1.png|thumb|600px|center|Fig 1. the signal of the mcherry fluorescence.]]
+[[File:T--HUBU-WUHAN--impro2.png|thumb|300px|center|Fig 2. the signal of the mcherry fluorescence]]
+[[File:T--HUBU-WUHAN--impro3.png|thumb|300px|center|Fig 3. the red fluorescence intensity and the OD 600nm of the cells]]
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>