Difference between revisions of "Part:BBa K3087000:Design"

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[[File:T--HK SKHLPSS-plasmidss.png|500px]]
 
[[File:T--HK SKHLPSS-plasmidss.png|500px]]
  
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The biobricks contained in our plasmid are illustrated in the above graph. Four basic parts we designed, including BBa_K3087000, BBa_K3087001,BBa_K3087002and BBa_K3087003, are the DNAs synthesized to form our Lactobacillus targeting nano-structure (LTN).
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The biobricks contained in our plasmid are illustrated in the above graph. Four basic parts we designed, including BBa_K3087000, BBa_K3087001,BBa_K3087002 and BBa_K3087003, are the DNAs synthesised to form our Lactobacillus targeting nano-structure (LTN).
  
 
The promoter used in these four basic parts is J23100 and the terminator used is B0054. HIV Terminal Binding Site (HTBS) used in each plasmid encode the end of our desired gene. The binding of HIV reverse transcriptase (HIVRT) would then reversely transcribe our target DNA.
 
The promoter used in these four basic parts is J23100 and the terminator used is B0054. HIV Terminal Binding Site (HTBS) used in each plasmid encode the end of our desired gene. The binding of HIV reverse transcriptase (HIVRT) would then reversely transcribe our target DNA.
  
  
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[[File:T--HK_SKHLPSS--validation.jpeg ‎|500px]]
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[[File:T--HK_SKHLPSS--validation.jpeg ‎|300px]]
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The validation is shown above.
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The validation of plasmid is shown above. O1, O2, O3 and O4 represent the part BBa_K3087000, BBa_K3087001, BBa_K3087002 and BBa_K3087003 respectively.  
  
 
The information of the four basic parts are as follow:
 
The information of the four basic parts are as follow:
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[[File:T--HK_SKHLPSS--parts_information.jpeg ‎|700px]]
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Basic Part Name Type                                     Description                                             Length(bp)
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BBa_K3087000 ssDNA Strand 1 for formation of Lactobacillus targeting nano-structure    304
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BBa_K3087001 ssDNA Strand 2 for formation of Lactobacillus targeting nano-structure  303
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BBa_K3087002 ssDNA Strand 3 for formation of Lactobacillus targeting nano-structure 312
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BBa_K3087003 ssDNA Strand 4 for formation of Lactobacillus targeting nano-structure 308
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===Source===
 
===Source===
  
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This is synthesized artificially based on the DNA sequence of Lactobacillus. It is not from any genome sequence.
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This is a newly synthesised sequence which is artificially designed based on the DNA sequence of Lactobacillus.
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===References===
 
===References===
  
 
https://parts.igem.org/wiki/index.php?title=Part:BBa_K3087000:Design&action=submit
 
https://parts.igem.org/wiki/index.php?title=Part:BBa_K3087000:Design&action=submit

Latest revision as of 03:02, 22 October 2019

_NOTOC_ Strand 1 for formation of Lactobacillus targeting nano-structure (LTN)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 13
    Illegal NheI site found at 36
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is needed to be complementary to other oligo from parts BBa_K3087001, BBa_K3087002 and BBa_K3087003 to form the Lactobacillus targeting nano-structure.

The design of the Lactobacillus targeting nano-structure plasmid are as follow:

T--HK SKHLPSS-plasmidss.png

The biobricks contained in our plasmid are illustrated in the above graph. Four basic parts we designed, including BBa_K3087000, BBa_K3087001,BBa_K3087002 and BBa_K3087003, are the DNAs synthesised to form our Lactobacillus targeting nano-structure (LTN).

The promoter used in these four basic parts is J23100 and the terminator used is B0054. HIV Terminal Binding Site (HTBS) used in each plasmid encode the end of our desired gene. The binding of HIV reverse transcriptase (HIVRT) would then reversely transcribe our target DNA.


T--HK SKHLPSS--validation.jpeg

The validation of plasmid is shown above. O1, O2, O3 and O4 represent the part BBa_K3087000, BBa_K3087001, BBa_K3087002 and BBa_K3087003 respectively.

The information of the four basic parts are as follow: T--HK SKHLPSS--parts information.jpeg

Source

This is a newly synthesised sequence which is artificially designed based on the DNA sequence of Lactobacillus.

References

https://parts.igem.org/wiki/index.php?title=Part:BBa_K3087000:Design&action=submit