Difference between revisions of "Part:BBa K3030000:Design"

 
 
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===Design Notes===
 
===Design Notes===
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We insected the sequence to the pcDNA 3.1+ plasmid as a vector, which ensures the normal function of EAAT2 in human astrocytes and neurons.
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We generated synonymous mutation in this part to ensure this protein to fit the RFC10 without leading errors in protein expression.
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===Source===
 
===Source===
  
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This part is derived from the Homo sapien.
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This part is derived from the Homo sapiens.
  
 
===References===
 
===References===

Latest revision as of 02:34, 22 October 2019


The EAAT2-coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We generated synonymous mutation in this part to ensure this protein to fit the RFC10 without leading errors in protein expression.

Source

This part is derived from the Homo sapiens.

References