Difference between revisions of "Part:BBa K3093001"
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ChbB promotor is the promotor of chitobiose operon. It has the NagC, ChbR and CAP binding sites and can be activated by N | ChbB promotor is the promotor of chitobiose operon. It has the NagC, ChbR and CAP binding sites and can be activated by N | ||
-acetylglucosamine disaccharide, chitobiose. [More information is available at EcoCyc: EG10143]. | -acetylglucosamine disaccharide, chitobiose. [More information is available at EcoCyc: EG10143]. | ||
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| + | The chbB promotor was obtained from E.coli MG1655 through PCR. And we remove the strong NagC binding site. | ||
Revision as of 02:32, 22 October 2019
chbB promoter
ChbB promotor is the promotor of chitobiose operon. It has the NagC, ChbR and CAP binding sites and can be activated by N -acetylglucosamine disaccharide, chitobiose. [More information is available at EcoCyc: EG10143].
The chbB promotor was obtained from E.coli MG1655 through PCR. And we remove the strong NagC binding site.
References
[1]Plumbridge J, Pellegrini O. Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP. Mol Microbiol. 2004 Apr;52(2):437-49. PubMed PMID: 15066032.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]