Difference between revisions of "Part:BBa K3192022:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
na
+
<p>This part DNA sequence was taken from NCBI and codon optimized for <i>E. coli</i> K12 chassis. The sequence was then reviewed for any illegal restriction sites and conservative edits were made as necessary. The coding sequence was then designed for Golden Gate (using Teselagen Software) assembly and then synthesized. This part was assembled into a larger composite part using Golden Gate Assembly and then sequenced for identity confirmation. </p>
−
 
+
  
  
 
===Source===
 
===Source===
  
−
Curpriavidus nectar
+
<i>Curpriavidus nectar</i>
  
 
===References===
 
===References===
 +
<p> <i>Cupriavidus necator</i> H16 PhaC1 (phaC1), PhaA (phaA), and PhaB1 (phaB1). (n.d.). Retrieved from https://www.ncbi.nlm.nih.gov/nuccore/MH558939.1.</p>

Latest revision as of 02:32, 22 October 2019


phaC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 564
    Illegal BamHI site found at 856
    Illegal BamHI site found at 1426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1063
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part DNA sequence was taken from NCBI and codon optimized for E. coli K12 chassis. The sequence was then reviewed for any illegal restriction sites and conservative edits were made as necessary. The coding sequence was then designed for Golden Gate (using Teselagen Software) assembly and then synthesized. This part was assembled into a larger composite part using Golden Gate Assembly and then sequenced for identity confirmation.


Source

Curpriavidus nectar

References

Cupriavidus necator H16 PhaC1 (phaC1), PhaA (phaA), and PhaB1 (phaB1). (n.d.). Retrieved from https://www.ncbi.nlm.nih.gov/nuccore/MH558939.1.