Difference between revisions of "Part:BBa K3153002:Design"
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===Source=== | ===Source=== | ||
− | oprF, the major outer membrane (OM) protein, comes from Pseudomonas aeruginosa | + | oprF, the major outer membrane (OM) protein, comes from Pseudomonas aeruginosa. fMT, an arsenic-chelating metallothionein, comes from a marine alga, Fucus vesiculosus |
− | fMT, an arsenic-chelating metallothionein, comes from a marine alga, Fucus vesiculosus | + | |
===References=== | ===References=== |
Latest revision as of 02:24, 22 October 2019
oprF-fMT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 608
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 697
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Based on the predicted secondary structure and information found in the literature, we chose 188aa(Val) as potential fusion sites for displaying fMT
Source
oprF, the major outer membrane (OM) protein, comes from Pseudomonas aeruginosa. fMT, an arsenic-chelating metallothionein, comes from a marine alga, Fucus vesiculosus