Difference between revisions of "Part:BBa K3153002:Design"

 
(Source)
 
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===Source===
 
===Source===
  
oprF, the major outer membrane (OM) protein, comes from Pseudomonas aeruginosa
+
oprF, the major outer membrane (OM) protein, comes from Pseudomonas aeruginosa. fMT, an arsenic-chelating metallothionein,  comes from a marine alga, Fucus vesiculosus
fMT, an arsenic-chelating metallothionein,  comes from a marine alga, Fucus vesiculosus
+
  
 
===References===
 
===References===

Latest revision as of 02:24, 22 October 2019


oprF-fMT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 608
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Based on the predicted secondary structure and information found in the literature, we chose 188aa(Val) as potential fusion sites for displaying fMT


Source

oprF, the major outer membrane (OM) protein, comes from Pseudomonas aeruginosa. fMT, an arsenic-chelating metallothionein, comes from a marine alga, Fucus vesiculosus

References