Difference between revisions of "Part:BBa K3002234"
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<partinfo>BBa_K3002234 short</partinfo> | <partinfo>BBa_K3002234 short</partinfo> | ||
− | This composite part contains a hygromycin resistance (BBa_K3002127) and the WT-PETase (BBa_K3002109) fused with an SP20HA-tag for easy detectionvia HA-antibody and enhanced secretion. | + | <html> |
+ | This composite part contains a hygromycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002127">BBa_K3002127</a>) and the WT-PETase (<a href="https://parts.igem.org/Part:BBa_K3002109">BBa_K3002109</a>) fused with an SP20HA-tag for easy detectionvia HA-antibody and enhanced secretion. | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 02:21, 22 October 2019
L2 hygromycin resistance 2 + cCA_Mut-PETase_SP20HA
This composite part contains a hygromycin resistance (BBa_K3002127) and the WT-PETase (BBa_K3002109) fused with an SP20HA-tag for easy detectionvia HA-antibody and enhanced secretion.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3453
Illegal PstI site found at 4426 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2633
Illegal PstI site found at 3453
Illegal PstI site found at 4426 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3453
Illegal PstI site found at 4426 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3453
Illegal PstI site found at 4426
Illegal NgoMIV site found at 1270
Illegal NgoMIV site found at 1452
Illegal NgoMIV site found at 3188
Illegal NgoMIV site found at 3215
Illegal NgoMIV site found at 4986 - 1000COMPATIBLE WITH RFC[1000]