Difference between revisions of "Part:BBa K3304105"
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This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG (part: ) wi initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD (part: ) and NTB (part: ) who conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB (part ) which constitutes the input for the downstream Join Gate (part ). The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions. | This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG (part: ) wi initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD (part: ) and NTB (part: ) who conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB (part ) which constitutes the input for the downstream Join Gate (part ). The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions. | ||
| − | https://2019.igem.org/wiki/images/6/60/T--Thessaloniki--results-f3.png | + | |
| + | https://2019.igem.org/wiki/images/c/ca/T--Thessaloniki--animation-step1.gif | ||
| + | https://2019.igem.org/wiki/images/0/02/T--Thessaloniki--animation-step2.gif | ||
| + | https://2019.igem.org/wiki/images/3/38/T--Thessaloniki--animation-step3.gifttps://2019.igem.org/wiki/images/6/60/T--Thessaloniki--results-f3.png | ||
https://2019.igem.org/wiki/images/7/70/T--Thessaloniki--results-f5.png | https://2019.igem.org/wiki/images/7/70/T--Thessaloniki--results-f5.png | ||
https://2019.igem.org/File:T--Thessaloniki--results-f12.png | https://2019.igem.org/File:T--Thessaloniki--results-f12.png | ||
Revision as of 02:20, 22 October 2019
Input Gate with ELK1 sequence
This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG (part: ) wi initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD (part: ) and NTB (part: ) who conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB (part ) which constitutes the input for the downstream Join Gate (part ). The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions.
https://2019.igem.org/File:T--Thessaloniki--results-f12.png
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]