Difference between revisions of "Part:BBa K3117040:Design"
 
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===Design Notes===
 
===Design Notes===
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For the creation of Fabs, CH1 was fused to a variable single chain fragment and the Igk light chain. The disulfid bonds that connects the two Fab fragments can be used to chemically conjugate two Fabs.
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The CH1 domain was fused to a single chain variable fragment against GPA33 to create a fab fragment.
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===Source===
 
===Source===

Latest revision as of 02:04, 22 October 2019


constant region 1 (CH1) of an antibody


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 105
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the creation of Fabs, CH1 was fused to a variable single chain fragment and the Igk light chain. The disulfid bonds that connects the two Fab fragments can be used to chemically conjugate two Fabs.

Source

CH1 originates from genomic DNA.

References