Difference between revisions of "Part:BBa K3002227"

Revision as of 01:58, 22 October 2019

L2 hygromycin resistance + ccA_Mut-PETase_SP20HA

This composite part contains a hygromycin resistance (BBa_K3002119) and the mutant PETase with the cCA (BBa_K3002109) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1789
Illegal PstI site found at 2873
Illegal PstI site found at 3846
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1789
Illegal NheI site found at 2053
Illegal PstI site found at 2873
Illegal PstI site found at 3846
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1789
Illegal BamHI site found at 1408
Illegal XhoI site found at 250
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1789
Illegal PstI site found at 2873
Illegal PstI site found at 3846
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1789
Illegal PstI site found at 2873
Illegal PstI site found at 3846
Illegal NgoMIV site found at 773
Illegal NgoMIV site found at 955
Illegal NgoMIV site found at 1326
Illegal NgoMIV site found at 2608
Illegal NgoMIV site found at 2635
Illegal NgoMIV site found at 4406
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3002227 short</partinfo>
-This composite part contains a hygromycin resistance (BBa_K3002119) and the mutant PETase with the cCA (BBa_K3002109) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
+<html>
+This composite part contains a hygromycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002119">BBa_K3002119</a>) and the mutant PETase with the cCA (<a href="https://parts.igem.org/Part:BBa_K3002109">BBa_K3002109</a>) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
+</html>
 <!-- Add more about the biology of this part here