Difference between revisions of "Part:BBa K3042005"

Revision as of 01:58, 22 October 2019

Dino III GFP Plasmid A dinoflagellate optimized reporter plasmid. DinoIII is a dinoflagellate expression system that is constructed on the basis of genomic and transcriptomic data from many dinoflagellates. DinoIII GFP is a dinoflagellate optimized reporter plasmid which was created by the University of Connecticut. This part can be used as a reporter via fluorescence. When the transformed cells fluoresce green, this indicates the expression of the GFP (Specher, 2019). This part has been successfully transformed into O.Marina by UNAM *insert a link to paper here *. iGEM GSU wants to replicate their results with this part as well as into Symbiodinium microadriaticum, another dinoflagellate. *All sequences/info are from snapgene* Features: RNA elements Sprecher, Brittany & Zhang, Huan & Lin, Senjie. (2019). Nuclear gene transformation in a dinoflagellate. 10.1101/602821. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 682
Illegal NheI site found at 1781
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1201
Illegal XhoI site found at 1102
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1763

AmpR promoter- Ampicillin resistance promoter AmpR- Product: Beta lactamase Confers resistance to ampicillin and related antibiotics

Ori- High copy number ColE1/pMB1/pBR322/pUC origin of replication Cap binding site-Cap binding activates transcription in the presence of cAMP

Lac operator- The lac repressor binds the lac operator to block transcription in E. Coli. This inhibition can be relieved by adding lactose or IPTG Lac promoter- Promoter for E. Coli lac operon Terminator- part k3042003 Cycle 3 GFP- Mammalian codon-optimized cycle 3 GFP (Crameri et al., 1996) Promoter region- part K3042002

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3042005 short</partinfo>
+<html>
 A dinoflagellate optimized reporter plasmid.
+DinoIII is a dinoflagellate expression system that is constructed on the basis of genomic and transcriptomic data from many dinoflagellates. DinoIII GFP is a dinoflagellate optimized reporter plasmid which was created by the University of Connecticut. This part can be used as a reporter via fluorescence. When the transformed cells fluoresce green, this indicates the expression of the GFP (Specher, 2019).
+	This part has been successfully transformed into O.Marina by UNAM *insert a link to paper here *.
+iGEM GSU wants to replicate their results with this part as well as into Symbiodinium microadriaticum, another dinoflagellate.
+*All sequences/info are from snapgene*
+Features:
+RNA elements
+Sprecher, Brittany & Zhang, Huan & Lin, Senjie. (2019). Nuclear gene transformation in a dinoflagellate. 10.1101/602821.
+</html>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
@@ Line 11: / Line 24: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3042005 SequenceAndFeatures</partinfo>
+AmpR promoter- Ampicillin resistance promoter
+AmpR- Product: Beta lactamase
+Confers resistance to ampicillin and related antibiotics
+Ori- High copy number ColE1/pMB1/pBR322/pUC origin of replication
+Cap binding site-Cap binding activates transcription in the presence of cAMP
+Lac operator- The lac repressor binds the lac operator to block transcription in E. Coli. This inhibition can be relieved by adding lactose or IPTG
+Lac promoter- Promoter for E. Coli lac operon
+Terminator- part k3042003
+Cycle 3 GFP- Mammalian codon-optimized cycle 3 GFP (Crameri et al., 1996)
+Promoter region- part K3042002
 <!-- Uncomment this to enable Functional Parameter display