Difference between revisions of "Part:BBa K117003:Design"
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These stop codons are translated as serine when supD is also transcribed. Polymerase is expressed only when both SupD and T7ptag mRNA are present. | These stop codons are translated as serine when supD is also transcribed. Polymerase is expressed only when both SupD and T7ptag mRNA are present. | ||
| − | + | The T7ptag was derived from the original AND gate obtained from Voigt lab. | |
===Source=== | ===Source=== | ||
Revision as of 01:32, 27 October 2008
T7ptag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
T7ptag is responsible for the transcription of T7 RNA polymerase. The polymerase gene has been modified to contain two amber stop codons (T7ptag).
These stop codons are translated as serine when supD is also transcribed. Polymerase is expressed only when both SupD and T7ptag mRNA are present.
The T7ptag was derived from the original AND gate obtained from Voigt lab.
Source
[http://www.voigtlab.ucsf.edu/index.html http://www.voigtlab.ucsf.edu/index.html]
References
Anderson JC, Voigt CA, Arkin AP (2007) [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1964800 Environmental signal integration by a modular AND gate], Molecular Systems Biology, 3 (133)