Difference between revisions of "Part:BBa K117003:Design"
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===References===
 
===References===
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Anderson JC, Voigt CA, Arkin AP (2007) [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1964800 Environmental signal integration by a modular AND gate], Molecular Systems Biology, 3 (133)
+
Anderson JC, Voigt CA, Arkin AP (2007) [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1964800 Environmental signal integration by a modular AND gate], Molecular Systems Biology, 3 (133)

Revision as of 01:29, 27 October 2008

T7ptag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7ptag is responsible for the transcription of T7 RNA polymerase. The polymerase gene has been modified to contain two amber stop codons (T7ptag).

These stop codons are translated as serine when supD is also transcribed. Polymerase is expressed only when both SupD and T7ptag mRNA are present.


Source

[http://www.voigtlab.ucsf.edu/index.html http://www.voigtlab.ucsf.edu/index.html]

References

Anderson JC, Voigt CA, Arkin AP (2007) [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1964800 Environmental signal integration by a modular AND gate], Molecular Systems Biology, 3 (133)