Difference between revisions of "Part:BBa K3042000"

Latest revision as of 01:48, 22 October 2019

Dinoflagellate Codon Optimized RFP

This part is a codon optimized version of RFP for use in use in Dinoflagellates, a single celled organism. This part was created to be used in DinoIII, a dinoflagellate backbone vector . The sequence for this part was synthesized via Snapgene. Considering dinoflagellates have preference when it comes to specific amino acids, the favorable codons of the dinoflagellates were chosen using the chart below to ensure the successful translation of the red fluorescent protein. This part can be used as a reporter/indicator of a successful transformation. Positive results are demonstrated by red fluorescence emitted from the cells.

Figure 1. Codon Optimized Chart for Oxyrrhis marina

This was the chart we used to design the RFP part in Snapgene, to be edited into the Dino III plasmid in place of the existing GFP part BBa_K3042005

mRFP1- monomeric derivative of Dsred (Campbell et al., 2002)

Brittany N. Sprecher, Huan Zhang, Senjie Lin (2019, April 9). Nuclear gene transformation in a dinoflagellate. doi: 10.1101/602821

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 13
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 696
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 13
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 13
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3042000 short</partinfo>
-This is a red fluorescent protein coding sequence that has been codon optimized for use in dinoflagellates.
+This part is a codon optimized version of RFP for use in use in Dinoflagellates, a single celled organism. This part was created to be used in DinoIII, a dinoflagellate backbone vector .  The sequence for this part was synthesized via Snapgene. Considering dinoflagellates have preference when it comes to specific  amino acids, the favorable codons of the dinoflagellates were chosen using the chart below to ensure the successful translation of the red fluorescent protein. This part can be used as a reporter/indicator of a successful transformation. Positive results are demonstrated by red fluorescence emitted from the cells.
+https://2019.igem.org/wiki/images/e/e9/T--Georgia_State--codonchart.png
+Figure 1. Codon Optimized Chart for Oxyrrhis marina
+This was the chart we used to design the RFP part in Snapgene, to be edited into the Dino III plasmid in place of the existing GFP part BBa_K3042005
+mRFP1- monomeric derivative of Dsred (Campbell et al., 2002)
+<p>Brittany N. Sprecher, Huan Zhang, Senjie Lin (2019, April 9). Nuclear gene transformation in a dinoflagellate. doi: 10.1101/602821</p>
 <!-- Add more about the biology of this part here