Difference between revisions of "Part:BBa K3002217"
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<partinfo>BBa_K3002217 short</partinfo> | <partinfo>BBa_K3002217 short</partinfo> | ||
− | This composite part contains a spectinomycin resistance (BBa_K3002102) and the mutant PETase with the cCA (BBa_K3002109) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion. | + | <html> |
+ | This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>) and the mutant PETase with the cCA (<a href="https://parts.igem.org/Part:BBa_K3002109">BBa_K3002109</a>) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion. | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 01:45, 22 October 2019
L2 spectinomycin resistance + cCA_Mut-PETase_SP20HA
This composite part contains a spectinomycin resistance (BBa_K3002102) and the mutant PETase with the cCA (BBa_K3002109) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2401
Illegal PstI site found at 3485
Illegal PstI site found at 4458 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2401
Illegal NheI site found at 2665
Illegal PstI site found at 3485
Illegal PstI site found at 4458 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2401
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2401
Illegal PstI site found at 3485
Illegal PstI site found at 4458 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2401
Illegal PstI site found at 3485
Illegal PstI site found at 4458
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3220
Illegal NgoMIV site found at 3247
Illegal NgoMIV site found at 5018 - 1000COMPATIBLE WITH RFC[1000]