Difference between revisions of "Part:BBa K3002215"

 
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<partinfo>BBa_K3002215 short</partinfo>
 
<partinfo>BBa_K3002215 short</partinfo>
  
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This composite part contains a spectinomycin resistance (BBa_K3002102) and the MHETase with the cCA (BBa_K3002114) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
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<html>
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This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>) and the MHETase with the cCA (<a href="https://parts.igem.org/Part:BBa_K3002114">BBa_K3002114</a>) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
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</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:42, 22 October 2019


L2 spectinomycin resistance + L1_connector_Pos2 + ccA_MHETase_SP20HA

This composite part contains a spectinomycin resistance (BBa_K3002102) and the MHETase with the cCA (BBa_K3002114) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2660
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
    Illegal NotI site found at 4073
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4983
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3671
    Illegal NgoMIV site found at 4132
    Illegal NgoMIV site found at 4162
    Illegal NgoMIV site found at 4477
  • 1000
    COMPATIBLE WITH RFC[1000]