Difference between revisions of "Part:BBa K3002214"
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This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>), the mutant PETase with the secretion signal GLE (<a href="https://parts.igem.org/Part:BBa_K3002110">BBa_K3002110</a>)  (BBa_K3002110) and the MHETase with the cCA (<a href="https://parts.igem.org/Part:BBa_K3002114">BBa_K3002114</a>)  (BBa_K3002114), both fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
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This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>), the mutant PETase with the secretion signal GLE (<a href="https://parts.igem.org/Part:BBa_K3002110">BBa_K3002110</a>) and the MHETase with the cCA (<a href="https://parts.igem.org/Part:BBa_K3002114">BBa_K3002114</a>), both fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
 
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Revision as of 01:41, 22 October 2019


L2 spectinomycin resistance + GLE_Mut-PETase_SP20HA + cCA_MHETase_SP20HA

This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal GLE (BBa_K3002110) and the MHETase with the cCA (BBa_K3002114), both fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5435
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6777
    Illegal PstI site found at 7101
    Illegal PstI site found at 7444
    Illegal PstI site found at 8254
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5435
    Illegal NheI site found at 2665
    Illegal NheI site found at 5699
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6777
    Illegal PstI site found at 7101
    Illegal PstI site found at 7444
    Illegal PstI site found at 8254
    Illegal NotI site found at 7112
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5435
    Illegal BglII site found at 8022
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5435
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6777
    Illegal PstI site found at 7101
    Illegal PstI site found at 7444
    Illegal PstI site found at 8254
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5435
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6777
    Illegal PstI site found at 7101
    Illegal PstI site found at 7444
    Illegal PstI site found at 8254
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3238
    Illegal NgoMIV site found at 3265
    Illegal NgoMIV site found at 5036
    Illegal NgoMIV site found at 6710
  • 1000
    COMPATIBLE WITH RFC[1000]