Difference between revisions of "Part:BBa K3002212"

 
Line 3: Line 3:
 
<partinfo>BBa_K3002212 short</partinfo>
 
<partinfo>BBa_K3002212 short</partinfo>
  
−
This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal cCA (BBa_K3002109) and the MHETase with the cCA (BBa_K3002114), both fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
+
<html>
 +
This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>), the mutant PETase with the secretion signal cCA (<a href="https://parts.igem.org/Part:BBa_K3002109">BBa_K3002109</a>) and the MHETase with the cCA (<a href="https://parts.igem.org/Part:BBa_K3002114">BBa_K3002114</a>), both fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
 +
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:37, 22 October 2019


L2 spectinomycin resistance + cCA_Mut-PETase_SP20HA + cCA_MHETase_SP20HA

This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal cCA (BBa_K3002109) and the MHETase with the cCA (BBa_K3002114), both fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5417
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6759
    Illegal PstI site found at 7083
    Illegal PstI site found at 7426
    Illegal PstI site found at 8236
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5417
    Illegal NheI site found at 2665
    Illegal NheI site found at 5681
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6759
    Illegal PstI site found at 7083
    Illegal PstI site found at 7426
    Illegal PstI site found at 8236
    Illegal NotI site found at 7094
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5417
    Illegal BglII site found at 8004
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5417
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6759
    Illegal PstI site found at 7083
    Illegal PstI site found at 7426
    Illegal PstI site found at 8236
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5417
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6759
    Illegal PstI site found at 7083
    Illegal PstI site found at 7426
    Illegal PstI site found at 8236
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3220
    Illegal NgoMIV site found at 3247
    Illegal NgoMIV site found at 5018
    Illegal NgoMIV site found at 6692
  • 1000
    COMPATIBLE WITH RFC[1000]