Difference between revisions of "Part:BBa K3117015:Design"
 
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===Design Notes===
 
===Design Notes===
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This part is codon optimized for CHO cells.
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Codon optimization was performed for mammalian expression.
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Latest revision as of 01:32, 22 October 2019


SpyTag codon optimized for CHO expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for CHO cells.


Source

SpyTag origins from genomic DNA.

References

1. Hatlem, Daniel; Trunk, Thomas; Linke, Dirk; Leo, Jack C. (2019): Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins. In: International journal of molecular sciences 20 (9). DOI: 10.3390/ijms20092129.