Difference between revisions of "Part:BBa K2950013"
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<partinfo>BBa_K2950013 short</partinfo> | <partinfo>BBa_K2950013 short</partinfo> | ||
| − | This is a part coding the sequence of laccase which is originally in the organism Rhus vernicifera. This laccase is responsible for the film formation of lacquer through the urushiol, water, and oxygen. This part is codon-optimized for use in E. coli | + | This is a part coding the sequence of laccase which is originally in the organism Rhus vernicifera. This laccase is responsible for the film formation of lacquer through the urushiol, water, and oxygen. This part is codon-optimized for use in E. coli. |
| + | In our study, we aim to extract the enzyme laccase to finish the experiment about our artificial lacquer. Therefore, we firstly transformed a plasmid with the expression of laccase downstream the lac-operator. Then, after the induction of IPTG, we began to extract this protein through its His-tag. Finally, we verified this protein through SDS-PAGE electrophoresis. Here is the result. | ||
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| + | <html> | ||
| + | <div style="text-align:center";> | ||
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| + | <img src="https://static.igem.org/mediawiki/parts/1/19/T--BNDS_China--OKS_ZhuI.png" width=600px></br> | ||
| + | <p style="width:600px; margin-left:150px; margin-bottom:60px; | ||
| + | text-align:center | ||
| + | "><em><strong>FIGURE 1</strong>The result of SDS-PAGE electrophoresis</em> </p> | ||
| + | <br> | ||
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| + | Because the length of this protein is 58981DA (about 59kDA), it should be between the 55kDA marker and 60kDA marker theoretically. According to figure 1, the mark of the sample is just between 55kDA marker and 60kDA marker, so this protein is produced and extracted successfully. Therefore, we finished the qualitative characterization of this part. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 01:30, 22 October 2019
laccase
This is a part coding the sequence of laccase which is originally in the organism Rhus vernicifera. This laccase is responsible for the film formation of lacquer through the urushiol, water, and oxygen. This part is codon-optimized for use in E. coli.
In our study, we aim to extract the enzyme laccase to finish the experiment about our artificial lacquer. Therefore, we firstly transformed a plasmid with the expression of laccase downstream the lac-operator. Then, after the induction of IPTG, we began to extract this protein through its His-tag. Finally, we verified this protein through SDS-PAGE electrophoresis. Here is the result.
FIGURE 1The result of SDS-PAGE electrophoresis
Because the length of this protein is 58981DA (about 59kDA), it should be between the 55kDA marker and 60kDA marker theoretically. According to figure 1, the mark of the sample is just between 55kDA marker and 60kDA marker, so this protein is produced and extracted successfully. Therefore, we finished the qualitative characterization of this part. Sequence and Features