Difference between revisions of "Part:BBa K3117012"

 
 
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<partinfo>BBa_K3117012 short</partinfo>
 
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pelB is a signal sequence, which can target recombinant proteins to the periplasmic space. It uses the post-translational translocation pathway (Singh et al. 2013).  
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===Usage and Biology===
 
===Usage and Biology===
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The secretion of the recombinant protein into the periplasma has distinct advantages like the oxidising environment for the formation of disulfide bonds, which does hardly occur in the reducing environment of the cytoplasm. Additionally, foldases inside the periplasm catalyse the formation and isomerisation of disulfide bonds (Schlegel et al. 2013).
  
 
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Latest revision as of 01:25, 22 October 2019


pelB signal sequence

pelB is a signal sequence, which can target recombinant proteins to the periplasmic space. It uses the post-translational translocation pathway (Singh et al. 2013).


Usage and Biology

The secretion of the recombinant protein into the periplasma has distinct advantages like the oxidising environment for the formation of disulfide bonds, which does hardly occur in the reducing environment of the cytoplasm. Additionally, foldases inside the periplasm catalyse the formation and isomerisation of disulfide bonds (Schlegel et al. 2013).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]