Difference between revisions of "Part:BBa K3019009"
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Pre-Ost1-pro-alfa-factor signal promotes translocation to the ER during translation. Arthrobacter sp. YCWD3 glcI gene for beta-1,3-glucanase, complete cds. Codon optimised for expression in S. cerevisiae. This glucanase is a part of Zymolyaze. | Pre-Ost1-pro-alfa-factor signal promotes translocation to the ER during translation. Arthrobacter sp. YCWD3 glcI gene for beta-1,3-glucanase, complete cds. Codon optimised for expression in S. cerevisiae. This glucanase is a part of Zymolyaze. | ||
| − | + | [[File:T--Tartu TUIT--Westernblot.png|500px]] | |
| − | + | Figure. Western Blot analysis to test the expression and secretion of Glc1. Western blot image showing the expression of α-3HA-Glc1 and Ost1-3HA-Glc1. The image shows the amount of the protein in cell lysate. The lysate was diluted 20 and 40 times to visualize the two fragments and to avoid signal leak to neighbouring lanes. | |
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Revision as of 01:23, 22 October 2019
Pre-Ost1-pro-α-factor signal 3HA GLCI
Pre-Ost1-pro-alfa-factor signal promotes translocation to the ER during translation. Arthrobacter sp. YCWD3 glcI gene for beta-1,3-glucanase, complete cds. Codon optimised for expression in S. cerevisiae. This glucanase is a part of Zymolyaze.
Figure. Western Blot analysis to test the expression and secretion of Glc1. Western blot image showing the expression of α-3HA-Glc1 and Ost1-3HA-Glc1. The image shows the amount of the protein in cell lysate. The lysate was diluted 20 and 40 times to visualize the two fragments and to avoid signal leak to neighbouring lanes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 645
Illegal AgeI site found at 1726 - 1000COMPATIBLE WITH RFC[1000]